Biotin Protein (Alkaline Phosphatase (AP))

Produktnummer ABIN964125

Hersteller Produkt- Nr.: b000-05

Dieses Biotin-Protein wird in Synthetic exprimiert.

Kurzübersicht für Biotin Protein (Alkaline Phosphatase (AP)) (ABIN964125)

Target: Biotin

Spezies: Chemical

Quelle: Synthetic

Applikation: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Biotin Protein ist gelabelt mit Alkaline Phosphatase (AP).

Hersteller Produkt- Nr.: b000-05

Hersteller: Rockland

Verwendungszweck: Biotin Alkaline Phosphatase Conjugated

Aufreinigung: This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Biotin

Substanzklasse: Chemical

Hintergrund:

Synonyms: Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav